The samples were centrifuged at 13000 g for 10 min at 4 C and the supernatant was collected and detected by HPLC to analyze the biostability of M-CuP and D-CuP against proteinase K
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Secondary endpoints included the proportion of participants achieving 5% and 10% body weight reduction, absolute weight change in kg, change in waist circumference, lipid parameters including total cholesterol, HDL, LDL, VLDL, triglycerides, and glycaemic variables such as HbA1c, fasting glucose, insulin, HOMA-IR, and -cell function
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The authors suggest an implication of the phosphorylated CREB, mediated by PI-3K and MAPK signaling pathways, that is known to decrease neurotoxicity and oxidative stress-induced neuronal death (Riccio et al., 1999
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